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ymfI [2018-02-10T13:08:59.000Z]
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ymfI [2018-02-10T13:08:59.000Z]

[protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp]-5 aminopentanone reductase
locus
BSU16870
pI
5.22
mw
25.43 kDa
protein length
242 aa Sequence Blast
gene length
726 bp Sequence Blast
function
modification of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp]
product
[protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp]-5 aminopentanone reductase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.14|Translation factor modification and maturation]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.8|Protein modification/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]

    • Gene

    Coordinates
    1,759,655 → 1,760,383

    Phenotypes of a mutant

  • defective in [category|SW 4.1.4|Swarming] motility [pubmed|28787546]

    • The protein

    Catalyzed reaction/ biological activity

  • reduction of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp]-5 aminopentanone to [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp]-5 aminopentanol [pubmed|28787546]

    • Paralogous protein(s)

  • [protein|B6FF689E65906186F3576B378650D713DB84EDDA|YcdF], [protein|439B468A13137000FB42E9389391CB4986FFED84|FabG] (34%), [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF], [protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|YhxC]

    • [SW|Cofactors]

  • NADPH [pubmed|28787546]

    • Structure

  • [PDB|2PNF] (from Aquifex Aeolicus , 36% identity)

    • Biological materials

    Mutant

  • MGNA-B379 (ymfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1378 NBRP B. subtilis, Japan]
  • BKE16870 (Δ[gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTCTTTCCTTCTTTCC, downstream forward: _UP4_TGATCCGAAAATATTCGGTG
  • BKK16870 (Δ[gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTCTTTCCTTCTTTCC, downstream forward: _UP4_TGATCCGAAAATATTCGGTG

    • References

    Research papers

  • 28787546